The two imaging modes, which to the best of our knowledge have not previously been combined, are complementary: light sheet fluorescence microscopy provides three- dimensional imaging of fluorescently labelled components of multicellular systems with high speed, large fields of view, and low phototoxicity, whereas differential interference contrast microscopy reveals the unlabelled neighbourhood of tissues, organs, and other structures with high contrast and inherent optical sectioning. Use of a single Nomarski prism for differential interference contrast microscopy and a shared detection path for both imaging modes enables simple integration of the two techniques in one custom microscope. We provide several examples of the utility of the resulting instrument, focusing especially on the digestive tract of the larval zebrafish, revealing in this complex and heterogeneous environment anatomical features, the behaviour of commensal microbes, immune cell motions, and more. PMID: 2. 56. 11. 32. A Simple low- cost device enables four epi- illumination techniques on standard lightmicroscopes. NASA Astrophysics Data System (ADS)Ishmukhametov, Robert R.; Russell, Aidan N.; Wheeler, Richard J.; Nord, Ashley L.; Berry, Richard M. Back- scattering darkfield (BSDF), epi- fluorescence (EF), interference reflection contrast (IRC), and darkfield surface reflection (DFSR) are advanced but expensive light microscopy techniques with limited availability.
Here we show a simple optical design that combines these four techniques in a simple low- cost miniature epi- illuminator, which inserts into the differential interference- contrast (DIC) slider bay of a commercial microscope, without further additions required. We demonstrate with this device: 1) BSDF- based detection of Malarial parasites inside unstained human erythrocytes; 2) EF imaging with and without dichroic components, including detection of DAPI- stained Leishmania parasite without using excitation or emission filters; 3) RIC of black lipid membranes and other thin films, and 4) DFSR of patterned opaque and transparent surfaces.
We believe that our design can expand the functionality of commercial bright field microscopes, provide easy field detection of parasites and be of interest to many users of light microscopy. The scanning transmission microscope at the NSLS (National Synchrotron Light Source)Sci.
Tech Connect. Rarback, H.; Buckley, C.; Goncz, K.; Ade, H.; Anderson, E.; Attwood, D.; Batson, P.; Hellman, S.; Jacobsen, C.; Kern, D.; Kirz, J.; Lindaas, S.; Mc. Nulty,I.; Oversluizen, M.; Rivers, M.; Rothman, S.; Shu, D.; Tang, Eshang; State Univ. Dep. 19. 89- 0. 1- 0.
- A tour round a Leitz Diaplan microscope by David Walker, UK Update: Willem Steurbaut has very kindly shared a Leitz brochure and Leitz article on the Diaplan which.
- This is database page 2. Please use the Library Index to access the article categories and links.
A Brief History of Leitz Microscope Manufacturing. In 1849, Karl Kellner founded the Optical Institute in Wetzlar, Germany. Leitz Microscope Features & Versatility Orthoplan, Aristoplan and Diaplan. The Leitz microscope traces its lineage to a company founded in 1849 in Wetzlar, Germany. I Description 15 12 DIAPLAN with Lamphousing 103 Z, UKO universal condenser, No, 87 mechanical stage, and binocular observation tube S. Diseases of female reproductive apparatus (see also physiology of female reproductive apparatus) diseases of breast / mastopathies (see also physiology of breast).
Light microscopic image analysis system to quantify immunoreactive terminal area apposed to nerve cells. NASA Technical Reports Server (NTRS) Wu, L.
The scanning transmission soft x- ray microscope (STXM), that has been under development at the National Synchrotron Light Source has been substantially upgraded for operation with the X1 undulator. The principal new features are: optical prefocusing, using a visible light interferometer, a dedicated VAXstation 3. MHz. In conjunction with new zone plates of better resolution and higher efficiency, the microscope is ready for a period of extended use in biological imaging. The Light Microscopy Module: An On- Orbit Multi- User Microscope Facility. NASA Technical Reports Server (NTRS)Motil, Susan M.; Snead, John H.
The Light Microscopy Module (LMM) is planned as a remotely controllable on- orbit microscope subrack facility, allowing flexible scheduling and operation of fluids and biology experiments within the Fluids and Combustion Facility (FCF) Fluids Integrated Rack (FIR) on the International Space Station (ISS). The LMM will be the first integrated payload with the FIR to conduct four fluid physics experiments. A description of the LMM diagnostic capabilities, including video microscopy, interferometry, laser tweezers, confocal, and spectrophotometry, will be provided.
Semi- automated 3. D Leaf Reconstruction and Analysis of Trichome Patterning from Light. Microscopic Images. Pub. Med Central.
Schrader, Andrea; H. They are known to be involved in pathogen resistance.
Their patterning is considered to emerge from a field of initially equivalent cells through the action of a gene regulatory network involving trichome fate promoting and inhibiting factors. For a quantitative analysis of single and double mutants or the phenotypic variation of patterns in different ecotypes, it is imperative to statistically evaluate the pattern reliably on a large number of leaves. Here we present a method that enables the analysis of trichome patterns at early developmental leaf stages and the automatic analysis of various spatial parameters.
We focus on the most challenging young leaf stages that require the analysis in three dimensions, as the leaves are typically not flat. Our software Trich. Eratops reconstructs 3.
D surface models from 2. D stacks of conventional light- microscope pictures. It allows the GUI- based annotation of different stages of trichome development, which can be analyzed with respect to their spatial distribution to capture trichome patterning events. We show that 3. D modeling removes biases of simpler 2. D models and that novel trichome patterning features increase the sensitivity for inter- accession comparisons.
PMID: 2. 36. 37. 58. D scanning of internal structure in gel engineering materials with visual scanning microscopiclight scattering. NASA Astrophysics Data System (ADS)Watanabe, Yosuke; Gong, Jing; Masato, Makino; Kabir, M. Hasnat; Furukawa, Hidemitsu.
The 3. D printing technology, causing much attention from the beginning of 2. Recently our group of Yamagata University has developed the world- first 3. D Gel Printer to fabricate the complicated gel- materials with high- strength and biocompatibility. However, there are no 3. D scanners that collect the data from the internal structure of complicated gel objects such as eye lens.
It means that a new system for scanning the internal structure is needed now. In this study, firstly, we have tried to investigate the gel network of synthetic and biological gel with scanning microscopiclight scattering (SMILS). We calculated the Young's modulus of synthetic gels with the SMILS and with the tensile test, and precisely compared the results between them.
The temperature dependences of the inside structure and the transparency are observed in the pig crystalline lens. The quantitative analysis indicates the importance of the internal structure of real object. Secondary, we show the new system named Gel- scanner that can provide the 2- dimentional data of the internal structure. From examining our findings, the scanning of internal structure will enable us to expect physical properties of the real object. We convince that the gelscanner will play major role in the various fields.
Lightmicroscopic studies of the stomach of the lesser mouse deer (Tragulus javanicus). Pub. Med. Agungpriyono, S; Yamada, J; Kitamura, N; Sigit, K; Yamamoto, Y; Winarto, A; Yamashita, T1.
The stomach of the lesser mouse deer was studied at the lightmicroscopic level using histological and immunohistochemical methods. The stomach was clearly differentiated into rumen, reticulum including reticular groove, a small transition zone and abomasum. The mucosal surface of the rumen, reticulum and transition zone was lined with a stratified squamous epithelium and that of the abomasum with a simple columnar type. The epithelial keratinization was weak in the rumen, floor of the reticular groove and transition zone, while it was strong in the reticulum, especially on the tip of the reticulum papillae. Large sinusoidal capillaries were often present in the ruminal papillae. In the ruminal mucosa, a thin layer of alpha- smooth muscle actin immunoreactive cells was demonstrated by immunohistochemistry.
The muscularis mucosae of the reticulum was continuous and well- developed. The transition zone appeared as a nonglandular area having many low mucosal folds and two layers of tunica muscularis. The abomasal mucosa consisted of cardiac, proper gastric and pyloric glands.
Cells immunoreactive for bovine pepsinogen and bovine prochymosin antisera were demonstrated in the abomasum. It is suggested that the characteristic features observed might be adaptations to a relatively rapid passage and rapid absorption of the fermentation products. There is some evidence that the transition zone is not a part of either the floor of the reticular groove or the abomasum, suggesting a possible reevaluation of the term used for the reticulo- abomasal orifice in the mouse deer. PMID: 7. 53. 60. 16. Quantitative evaluation of a handheld lightmicroscope for field diagnosis of soil- transmitted helminth infection. Pub. Med. Bogoch, Isaac I; Andrews, Jason R; Speich, Benjamin; Ame, Shaali M; Ali, Said M; Stothard, J Russell; Utzinger, J.
A total of 9. 1 Kato- Katz thick smears were examined by experienced microscopists and helminth eggs were counted and expressed as eggs per gram of stool (EPG). Mean egg counts were significantly higher with the conventional lightmicroscope (5,1. EPG versus 2,3. 86 EPG for Ascaris lumbricoides; 8. Trichuris trichiura; both P < 0. Using regression coefficients and accounting for intensity of infection, we found that the agreement between the two devices was excellent for both species (. The Newton Nm. 1 microscope may be a useful tool for the detection and quantification of soil- transmitted helminth infection in clinical, epidemiologic, and public health settings.
ICLs were morphologically divided into two types. Type A was characterized by the presence of secretory materials stained with eosin in the lumen and Type B by the cytoplasmic vacuoles under lightmicroscope. Electron microscopic observation on Type A ICLs showed numerous filiform microvilli projecting towards the lumen and various amounts of secretory materials in the lumen. Type B of ICLs only had scanty and short microvilli and rarely secretory materials in the lumen. The results indicated that: 1.